ER domains

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ER domains

n page 897, Luedeke et al. show that, like the plasma membrane, the ER can be compartmentalized into distinct membrane domains. In budding yeast, this separation restricts the diffusion of ER membrane proteins between the bud and the mother cell. The mRNAs of many bud-specific membrane proteins are actively transported into the bud. To keep these mRNAs and proteins in the bud, they must be kept...

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A 3D analysis of yeast ER structure reveals how ER domains are organized by membrane curvature

We analyzed the structure of yeast endoplasmic reticulum (ER) during six sequential stages of budding by electron tomography to reveal a three-dimensional portrait of ER organization during inheritance at a nanometer resolution. We have determined the distribution, dimensions, and ribosome densities of structurally distinct but continuous ER domains during multiple stages of budding with and wi...

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ER-to-Golgi carriers arise through direct en bloc protrusion and multistage maturation of specialized ER exit domains.

Protein transport between the ER and the Golgi in mammalian cells occurs via large pleiomorphic carriers, and most current models suggest that these are formed by the fusion of small ER-derived COPII vesicles. We have examined the dynamics and structural features of these carriers during and after their formation from the ER by correlative video/light electron microscopy and tomography. We foun...

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High-curvature domains of the ER are important for the organization of ER exit sites in Saccharomyces cerevisiae.

Protein export from the endoplasmic reticulum (ER) to the Golgi apparatus occurs at specialized regions known as the ER exit sites (ERES). In Saccharomyces cerevisiae, ERES appear as numerous scattered puncta throughout the ER. We examined ERES within the peripheral ER, finding that the proteins comprising the ERES localize on high-curvature ER domains where curvature-stabilizing protein Rtn1 i...

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On weakly eR-open functions

The main goal of this paper is to introduce and study a new class of function via the notions of $e$-$theta$-open sets and $e$-$theta$-closure operator which are defined by Özkoç and Aslım [10] called weakly $eR$-open functions and $e$-$theta$-open functions. Moreover, we investigate not only some of their basic properties but also their relationships with other types of already existing topolo...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2005

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb1696iti1